Figure 7.
Effect of eritoran tetrasodium on corneal inflammation induced by antibiotic-killed P. aeruginosa. P. aeruginosa strain ATCC 19660 was killed by 30 minutes incubation with 0.3% tobramycin, and 1 × 107 bacteria were added to the abraded corneal surface of C57BL/6, TLR4−/−, and MD-2−/− mice, and a 2 mm diameter punch from a silicon hydrogel was added. Corneas were treated with 2.2 µg eritoran tetrasodium in 2 µL H2O or with placebo. After 24 hours, neutrophil infiltration, and corneal thickness and haze were measured as before. Data were analyzed by ANOVA and statistically significant differences (*P < 0.05; **P < 0.001) were noted for all parameters between C57BL/6 mice compared with TLR4−/− and MD-2−/− mice (A), and between eritoran tetrasodium compared with placebo. These data are the mean ± SD of five mice per group, and the experiments were repeated twice with similar results.