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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Mol Biosyst. 2014 Jan;10(1):45–53. doi: 10.1039/c3mb70417c

Figure 1.

Figure 1

Schematic overview. Wnt signalling was activated in three different human cell-lines (HEK293T, HCT116 and RKO) by treatment with Wnt3A ligand. Protein samples extracted from the treated cells were prepared for global protein expression analysis using either label free LC-MS/MS or immune blots with native antibodies as shown. Affinity-purification mass-spectrometry (AP-MS) was used to characterize β-catenin associated proteins in the same cell lines. Protein sub-networks were then generated according to individual cell line.