Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1985 Dec;82(24):8535–8539. doi: 10.1073/pnas.82.24.8535

Expression of the cytokeratin endo A gene during early mouse embryogenesis.

P Duprey, D Morello, M Vasseur, C Babinet, H Condamine, P Brûlet, F Jacob
PMCID: PMC390951  PMID: 2417224

Abstract

Expression of cytokeratin endo A has been analyzed during mouse blastocyst formation and embryonal carcinoma cell differentiation. To study the regulation of endo A expression, nuclease S1 mapping experiments have been performed on RNA extracted from two-cell to 7.5-day embryos. Low levels of endo A mRNA begin to be detectable in eight-cell embryos. The amount of this mRNA increases at the blastocyst stage, suggesting that endo A expression is regulated at the mRNA level during blastocyst formation. At this stage, in situ hybridization studies show that endo A mRNA is present in the trophectoderm but not in the inner cell mass. In 7.5-day embryos, endo A mRNAs are also detectable in the endoderm layer and in the amnion.

Full text

PDF
8535

Images in this article

8536Image
on p.8536
8537Image
on p.8537
8537Image
on p.8537
8537Image
on p.8537
8537Image
on p.8537
8537Image
on p.8537
8538Image
on p.8538

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Todaro G. J. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J Cell Physiol. 1968 Oct;72(2):141–148. doi: 10.1002/jcp.1040720208. [DOI] [PubMed] [Google Scholar]
  2. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  3. Battey J., Moulding C., Taub R., Murphy W., Stewart T., Potter H., Lenoir G., Leder P. The human c-myc oncogene: structural consequences of translocation into the IgH locus in Burkitt lymphoma. Cell. 1983 Oct;34(3):779–787. doi: 10.1016/0092-8674(83)90534-2. [DOI] [PubMed] [Google Scholar]
  4. Brûlet P., Babinet C., Kemler R., Jacob F. Monoclonal antibodies against trophectoderm-specific markers during mouse blastocyst formation. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4113–4117. doi: 10.1073/pnas.77.7.4113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brûlet P., Condamine H., Jacob F. Spatial distribution of transcripts of the long repeated ETn sequence during early mouse embryogenesis. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2054–2058. doi: 10.1073/pnas.82.7.2054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brûlet P., Jacob F. Molecular cloning of a cDNA sequence encoding a trophectoderm-specific marker during mouse blastocyst formation. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2328–2332. doi: 10.1073/pnas.79.7.2328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clegg K. B., Pikó L. Poly(A) length, cytoplasmic adenylation and synthesis of poly(A)+ RNA in early mouse embryos. Dev Biol. 1983 Feb;95(2):331–341. doi: 10.1016/0012-1606(83)90034-9. [DOI] [PubMed] [Google Scholar]
  8. Derman E., Krauter K., Walling L., Weinberger C., Ray M., Darnell J. E., Jr Transcriptional control in the production of liver-specific mRNAs. Cell. 1981 Mar;23(3):731–739. doi: 10.1016/0092-8674(81)90436-0. [DOI] [PubMed] [Google Scholar]
  9. Franke W. W., Schmid E., Schiller D. L., Winter S., Jarasch E. D., Moll R., Denk H., Jackson B. W., Illmensee K. Differentiation-related patterns of expression of proteins of intermediate-size filaments in tissues and cultured cells. Cold Spring Harb Symp Quant Biol. 1982;46(Pt 1):431–453. doi: 10.1101/sqb.1982.046.01.041. [DOI] [PubMed] [Google Scholar]
  10. Jackson B. W., Grund C., Schmid E., Bürki K., Franke W. W., Illmensee K. Formation of cytoskeletal elements during mouse embryogenesis. Intermediate filaments of the cytokeratin type and desmosomes in preimplantation embryos. Differentiation. 1980;17(3):161–179. doi: 10.1111/j.1432-0436.1980.tb01093.x. [DOI] [PubMed] [Google Scholar]
  11. Jackson B. W., Grund C., Winter S., Franke W. W., Illmensee K. Formation of cytoskeletal elements during mouse embryogenesis. II. Epithelial differentiation and intermediate-sized filaments in early postimplantation embryos. Differentiation. 1981;20(3):203–216. doi: 10.1111/j.1432-0436.1981.tb01177.x. [DOI] [PubMed] [Google Scholar]
  12. Jakob H., Boon T., Gaillard J., Nicolas J., Jacob F. Tératocarcinome de la spuris: isolement, culture et propriétés de cellules a potentialités multiples. Ann Microbiol (Paris) 1973 Oct;124(3):269–282. [PubMed] [Google Scholar]
  13. Kemler R., Brûlet P., Schnebelen M. T., Gaillard J., Jacob F. Reactivity of monoclonal antibodies against intermediate filament proteins during embryonic development. J Embryol Exp Morphol. 1981 Aug;64:45–60. [PubMed] [Google Scholar]
  14. Nicolas J. F., Avner P., Gaillard J., Guenet J. L., Jakob H., Jacob F. Cell lines derived from teratocarcinomas. Cancer Res. 1976 Nov;36(11 Pt 2):4224–4231. [PubMed] [Google Scholar]
  15. Oshima R. G. Developmental expression of murine extra-embryonic endodermal cytoskeletal proteins. J Biol Chem. 1982 Apr 10;257(7):3414–3421. [PubMed] [Google Scholar]
  16. Oshima R. G., Howe W. E., Klier F. G., Adamson E. D., Shevinsky L. H. Intermediate filament protein synthesis in preimplantation murine embryos. Dev Biol. 1983 Oct;99(2):447–455. doi: 10.1016/0012-1606(83)90294-4. [DOI] [PubMed] [Google Scholar]
  17. Paulin D., Babinet C., Weber K., Osborn M. Antibodies as probes of cellular differentiation and cytoskeletal organization in the mouse blastocyst. Exp Cell Res. 1980 Dec;130(2):297–304. doi: 10.1016/0014-4827(80)90006-3. [DOI] [PubMed] [Google Scholar]
  18. Pikó L., Clegg K. B. Quantitative changes in total RNA, total poly(A), and ribosomes in early mouse embryos. Dev Biol. 1982 Feb;89(2):362–378. doi: 10.1016/0012-1606(82)90325-6. [DOI] [PubMed] [Google Scholar]
  19. Strickland S., Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell. 1978 Oct;15(2):393–403. doi: 10.1016/0092-8674(78)90008-9. [DOI] [PubMed] [Google Scholar]
  20. Vasseur M., Duprey P., Brûlet P., Jacob F. One gene and one pseudogene for the cytokeratin endo A. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1155–1159. doi: 10.1073/pnas.82.4.1155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ziomek C. A., Johnson M. H. Cell surface interaction induces polarization of mouse 8-cell blastomeres at compaction. Cell. 1980 Oct;21(3):935–942. doi: 10.1016/0092-8674(80)90457-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES