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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Cell Biochem. 2013 Nov 2;387(0):113–121. doi: 10.1007/s11010-013-1876-3

Figure 4.

Figure 4

a: Purification of 17.5kDa WGA Binding Polypeptide. Silver stained 12% SDS-PAGE (lanes 1–2). Lectin blot stained with biotinylated WGA (lanes 3–4). Lanes 1and 3: 0.4 M NaCl eluted fraction of DEAE- Sepharose Chromatography of Triton X-100 soluble fraction of bovine cauda sperm plasma membrane. Lanes 2 and 4: Pooled fractions of continuous elution SDS-PAGE containing17.5kDa WGA binding polypeptide. The purified 17.5kDa WGA binding polypeptide was subjected to proteomic analysis.

b: Tryptic peptides of the 17.5 kDa polypeptide identified by MALDI-TOF-TOF proteomic analysis