Fate of 17.5kDa WGA Binding Polypeptide during Capacitation and Acrosome Reaction. Lectin blots stained with biotinylated WGA revealed the retention of several polypeptides in pelleted sperm fractions, including the 17.5kDa protein (lanes 2, 4, 6, and 8). Thus, the 17.5kDa WGA binding polypeptide is retained during capacitation and acrosome reaction and therefore must be integrated within the structure of a sperm cell’s plasma membrane.