Table 1.

Blood chemistry in mice fed diets supplemented with ellagic acid and/or embelin

		Control	EA	EM	EA+EM
Albumin	g/dL	2.1±0.1	2.1±0.0	2.2±0.1	2.3±0.1
T-bilirubin	mg/dL	0.2±0.0	0.2±0.0	0.2±0.0	0.2±0.0
Gamma glutamyl Transferase (GGT)	U/L	10.3±2.1	7.5±1.1	9.5±1.8	9.8±1.2
Protein-total	g/dL	4.6±0.2	4.7±0.1	4.7±0.1	5.0±0.2
Blood urea nitrogen (BUN)	mg/dL	15.3±1.5	19.0±0.8	20.5±1.5	22.2±1.2
Creatinine	mg/dL	0.2±0.0	0.3±0.0	0.3±0.0	0.3±0.0
Alanine aminotransferase (ALT)	U/L	24.3±3.2	45.2±15.9	24.8±2.3	33.8±3.9
Alkaline phosphatase (ALP)	U/L	53.0±3.2	68.5±4.8	59.7±3.5	72.5±5.1
Aspartate aminotransferase (AST)	U/L	188.0±51.2	154.0±28.4	128.2±24.6	116.3±8.6
Lactate dehydrogenase (LDH)	U/L	714.3±235.5	473.8±129.2	465.7±141.1	347.7±79.3

Data: mean±SEM (n=6)

Nude male mice were fed for 6 weeks control diets (control) or diets supplemented with ellagic acid (EA), embelin (EM), or a combination of ellagic acid and embelin (EA+EM). Xenograft s.c. tumors were implanted in each mouse one week after diet initiation. Mice were kept on diets for 5 more weeks and then sacrificed. Table shows blood chemistry for the selected parameters at sacrifice. Values represent mean ± SEM of 6 animals in each group; there are no significant differences between groups (Bonferroni t test).

All blood values were within normal range, indicating normal liver and kidney function. These data suggest that the phytochemicals were well tolerated and did not induce apparent toxicity.