Figure 5. AZ58 treatment results in reduced tumor burden; however, in a non-statistically significant fashion. (A) Microscopy of UMUC-6 cells demonstrating differential cell death in the various treatment conditions as labeled. Corresponding images show resultant graphs of PI-FACS demonstrating DNA fragmentation. (B) Female athymic nude mice were injected with UMUC-6 cells and treated as described in Materials and Methods. Tumor volumes were calculated and plotted. n = 6 mice for each condition. Error bars = standard error. (C) TUNEL staining of fragmented apoptotic DNA/nuclei to detect the efficacy of AZ58 and chemotherapy in vivo (green) and the total nuclei stained using propidium iodide (Red). Magnification 10 ×. (D) Mean number of TUNEL positive nuclei from 10 representative images.