Figure 5. Dynamics of histone GFP-H1.1 by FRAP. (A) FRAP of GFP-H1.1 in live HeLa cells exposed to 500 nM daunomycin for 0.5 h, indicating the presence of a mobile pool (open symbols, untreated; closed symbols, daunomycin-treated). (B) Dynamics of histone GFP-H1.1 returns to the level of untreated cells after 4 h incubation with daunomycin (500 nM). (C) Dynamics of four point mutants of GFP-H1.1, in 3T3 fibroblasts exposed to daunomycin (500 nM, for 4 h; open symbols, untreated; closed symbols, daunomycin-treated). Note a different time scale in graphs representing mutants R42A and K69A characterized by a rapid rate of exchange.