Figure 2. Phospho-Met expression is specific to the p53^R175H. (A) Panel of four different p53 mutations R175H, R273H, R248W, and V143A were analyzed by western blotting for phospho-Met expression. Only the cells expressing the p53^R175H mutant have increased phospho-Met expression. (B) Mouse esophageal keratinocytes (MEK) isolated from p53 null mice (MEK3N p53K puro, MEK2N p53K) and wild-type p53 mice (MEK3N, MEK3N puro) were transfected with human p53^R175H, in both the p53 null (MEK2 p53K p53^R175H) and wt p53 (MEK3N p53^R175H). The p53^R175H did not have the same effect on activation of phospho-Met as in human keratinocytes.