Table 2.
Experimental design matrix for optimization of chitin deacetylase production using central composite design
| Run | Independent variables (actual values) | CDA (Ul−1) | ||||
|---|---|---|---|---|---|---|
| pH | Temperature (°C) | Inoculum age (h) | Inoculum size (%) | Observed | Predicted | |
| 1 | 5.0 | 25.0 | 96.0 | 4.0 | 527.85 ± 3.2 | 520.75 |
| 2 | 5.0 | 25.0 | 96.0 | 10.0 | 543.16 ± 4.1 | 545.17 |
| 3 | 5.0 | 25.0 | 144.0 | 4.0 | 475.32 ± 2.8 | 478.63 |
| 4 | 5.0 | 25.0 | 144.0 | 10.0 | 528.73 ± 2.5 | 523.42 |
| 5 | 5.0 | 35.0 | 96.0 | 4.0 | 559.26 ± 3.4 | 550.96 |
| 6 | 5.0 | 35.0 | 96.0 | 10.0 | 558.14 ± 2.1 | 552.75 |
| 7 | 5.0 | 35.0 | 144.0 | 4.0 | 490.25 ± 3.1 | 491.08 |
| 8 | 5.0 | 35.0 | 144.0 | 10.0 | 516.35 ± 2.7 | 513.13 |
| 9 | 7.0 | 25.0 | 96.0 | 4.0 | 539.03 ± 1.8 | 545.96 |
| 10 | 7.0 | 25.0 | 96.0 | 10.0 | 568.34 ± 2.3 | 568.75 |
| 11 | 7.0 | 25.0 | 144.0 | 4.0 | 513.23 ± 3.4 | 520.08 |
| 12 | 7.0 | 25.0 | 144.0 | 10.0 | 551.76 ± 2.9 | 563.13 |
| 13 | 7.0 | 35.0 | 96.0 | 4.0 | 581.28 ± 3.3 | 587.42 |
| 14 | 7.0 | 35.0 | 96.0 | 10.0 | 587.87 ± 4.1 | 587.46 |
| 15 | 7.0 | 35.0 | 144.0 | 4.0 | 542.84 ± 3.8 | 545.17 |
| 16 | 7.0 | 35.0 | 144.0 | 10.0 | 556.54 ± 3.4 | 564.08 |
| 17 | 8.0 | 30.0 | 120.0 | 7.0 | 629.74 ± 2.7 | 610.13 |
| 18 | 4.0 | 30.0 | 120.0 | 7.0 | 521.71 ± 2.5 | 533.96 |
| 19 | 6.0 | 40.0 | 120.0 | 7.0 | 540.65 ± 2.4 | 542.13 |
| 20 | 6.0 | 20.0 | 120.0 | 7.0 | 519.85 ± 3.2 | 510.96 |
| 21 | 6.0 | 30.0 | 168.0 | 7.0 | 472.16 ± 1.7 | 461.79 |
| 22 | 6.0 | 30.0 | 72.0 | 7.0 | 523.37 ± 2.4 | 527.29 |
| 23 | 6.0 | 30.0 | 120.0 | 13.0 | 596.39 ± 3.5 | 593.46 |
| 24 | 6.0 | 30.0 | 120.0 | 1.0 | 552.75 ± 2.6 | 548.63 |
| 25 | 6.0 | 30.0 | 120.0 | 7.0 | 546.24 ± 2.3 | 545.17 |
| 26 | 6.0 | 30.0 | 120.0 | 7.0 | 542.08 ± 3.1 | 545.17 |
| 27 | 6.0 | 30.0 | 120.0 | 7.0 | 543.61 ± 1.6 | 545.17 |
| 28 | 6.0 | 30.0 | 120.0 | 7.0 | 545.29 ± 3.5 | 545.17 |
| 29 | 6.0 | 30.0 | 120.0 | 7.0 | 547.66 ± 2.4 | 545.17 |
| 30 | 6.0 | 30.0 | 120.0 | 7.0 | 548.15 ± 2.6 | 545.17 |