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Acridine orange/ethidium bromide fluorescent staining of MDA-MB-231 cells for determine apoptosis: (a) DMSO 1% as control; (b) cells treated with IC50 concentration of compound 4a (c) cells treated with IC50 concentration of etoposide as positive control for 24 hr. White arrow indicates live cells and dashed arrow indicates apoptotic cells. The images of cells were taken with a fluorescence microscope at 400×
