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. Author manuscript; available in PMC: 2014 Feb 2.
Published in final edited form as: Antiviral Res. 2010 Apr 2;87(1):1–8. doi: 10.1016/j.antiviral.2010.03.013

Figure 4.

Figure 4

Anguizole alters the subcellular distribution of NS4B-GFP in transiently transfected cells. Huh7.5 cells were cultured in the absence (A, D, F) or presence (B, C, E, G) of 5 µM anguizole, either alone (F, G) or following transfection with NS4B-GFP (A, B, C) or GFP-NS5A (D, E). (A) In nontreated cells, NS4B-GFP displays an ER-associated pattern of localization, along with several membrane-associated foci (MAF) of varying size throughout the cytoplasm. (B and C) In the presence of anguizole, NS4B-GFP appears to form elongated, curved structures (“snakes”), most commonly observed to be small and dispersed (B), but occasionally observed to be dramatically long (C). (Insets of A and B) Zoomed-in images highlight the differences in shape and length between MAF and snakes. (D and E) The distribution pattern of a GFP-NS5A fusion protein is equivalent in untreated (D) and treated (E) samples. (F and G) The pattern of calnexin staining is equivalent in untreated (F) and treated cells (G). Scale bars represent 50 µm.