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. Author manuscript; available in PMC: 2014 Feb 2.
Published in final edited form as: Antiviral Res. 2010 Apr 2;87(1):1–8. doi: 10.1016/j.antiviral.2010.03.013

Figure 6.

Figure 6

Anguizole interacts with the second amphipathic helix (AH2) of NS4B. Extruded synthetic vesicles composed of 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphocholine (POPC) lipids were incubated with synthetic peptide comprising the AH2 region of NS4B and with either anguizole or clemizole hydrochloride. Dynamic light scattering was employed to measure the effective diameter of vesicles in a sample. (A – D) The size distribution of vesicle diameters is shown (gray bars), as well as a gaussian fit of the data (black dashed line). Samples contained (A) 300 µM POPC lipid vesicles only, (B) POPC vesicles + 13 µM AH2 peptide, (C) POPC vesicles + AH2 peptide + 26 µM anguizole, and (D) POPC vesicles + AH2 peptide + 26 µM clemizole hydrochloride, as a negative control compound. The x-axis is presented on a logarithmic scale. (E) The mean values of the effective vesicle diameters in at least 3 independent experiments are shown for all four samples. Error bars indicate SEM.