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. Author manuscript; available in PMC: 2014 Feb 2.
Published in final edited form as: J Cell Biochem. 2013 Oct;114(10):2284–2292. doi: 10.1002/jcb.24573

Figure 1. Lysine 158 is required for H. pylori-induced ubiquitination and activation of TAK1.

Figure 1

(A) Wild-type (WT) or K158R mutant TAK1 were expressed with K63-only ubiquitin and CagA in HEK293T cells. Flag immunoprecipitates from the lysates were immunoblotted for HA-ubiquitin. (B) WT or K158R mutant TAK1 were ectopically expressed in AGS cells, which were then infected with H. pylori as indicated. Flag immunoprecipitates from the lysates were immunoblotted for ubiquitin. (C) HEK293T cells were transfected with IL-8-luciferase reporter and either WT or K158R TAK1 and increasing amounts of CagA plasmids as indicated. Luciferase activity was measured 30 hrs post-transfection.