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. Author manuscript; available in PMC: 2014 Feb 2.
Published in final edited form as: J Cell Biochem. 2013 Oct;114(10):2284–2292. doi: 10.1002/jcb.24573

Figure 2. Ubiquitination at lysine 158 of TAK1 is important for its kinase activity.

Figure 2

(A) WT or K158R TAK1 were expressed in HEK293T cells with or without CagA. Flag-TAK1 immunoprecipitates were immunoblotted for phosphorylated TAK1. (B) WT or K158R TAK1 were expressed in HEK293T cells with or without CagA. Flag-TAK1 immunoprecipitates were subjected to an in vitro kinase assay using His-MKK6(K82A) as a substrate. Levels of phosphorylated MKK6, TAK1, and MKK6 are shown. (C) AGS cells expressing WT or K158R TAK1 were infected with H. pylori for indicated times. Flag-TAK1 immunoprecipitates were immunoblotted for phosphorylated TAK1. (D) AGS cells expressing WT or K158R TAK1 were infected with H. pylori for indicated times. Flag-TAK1 immunoprecipitates were subjected to an in vitro kinase assay as in (B).