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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Dec 25;1831(4):776–791. doi: 10.1016/j.bbalip.2012.12.007

Figure 4.

Figure 4

Yeast coq8, coq9, and coq10 mutants are hypersensitive to treatment with αLnn. Yeast strains were grown in YPD media and harvested during mid-log phase (0.2-1.0 OD600nm). The cor1 and atp2 null mutants serve as respiratory deficient controls. Cells were washed twice with sterile water and resuspended in phosphate buffer to a final OD600nm of 0.2. The designated fatty acids (final concentration of 200 μM) were added to a flask containing 20 ml of yeast /phosphate buffer as described in Section 2.6. Samples were removed either before addition of fatty acids (0 hour control) or after 2 hr of incubation at 30 °C in the presence of the designated fatty acid. Serial dilutions (1:5) were prepared and 2 μl were spotted onto YPD plate medium. Images were taken after two days of growth at 30 °C.