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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Dec 25;1831(4):776–791. doi: 10.1016/j.bbalip.2012.12.007

Figure 5.

Figure 5

Yeast coq10 null mutants expressing Coq8p, Coq10p, or CC1736 are resistant to treatment with αLnn. The fatty acid sensitivity assay was performed as described in Fig. 4 except three 100 μL aliquots were removed at 9 h of either no treatment or 200 μM αLnn. Dilutions were prepared, and then spread onto SD-complete or SD−Ura plate medium. The chart shows the number of colony forming units (CFU) of untreated (black) and αLnn-treated (white) yeast cells. Yeast coq3Δ, coq10Δ, and coq10Δ null mutants harboring empty vector are profoundly sensitivity to PUFA treatment as compared to PUFA treated wild-type yeast as determined by the two-sample t test; a, p < 4.3 E−07. Yeast coq10 null mutants expressing Coq8p, Coq10p, or CC1736 are resistant to PUFA treatment as compared to coq10 null mutants expressing empty vector; b, p < 2.6 E−04.