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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Biomaterials. 2013 Oct 16;35(1):196–205. doi: 10.1016/j.biomaterials.2013.09.105

Figure 5.

Figure 5

In-vivo animal model to test growth factor resistance by subcutaneously implanting alginate gels with growth factors. Alginate gels containing PBS, FGF-2, VEGF-A and PDGF-CC were subcutaneously implanted on the back of mice. The gels were harvested after seven days and immediately macroscopically imaged (A). Vascularity on the gel was determined for the macroscopic images using Metamorph (B). The gels were then frozen, sectioned and H&E stained (C). The thickness of the vascular layer was quantified by analyzing the H&E stained images using Metamorph (D). The sections were immunostained with anti-PECAM antibody and imaged with an epifluorescence microscope (E). Panel A - size bar = 3mm. Mag. size bar = 1mm. Panel B - size bar = 1mm. Mag. size bar = 250μm. Panel E - size bar = 250μm. *Statistically different from Ob/Ob group (p < 0.05). n=6