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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Biomaterials. 2013 Oct 16;35(1):196–205. doi: 10.1016/j.biomaterials.2013.09.105

Figure 6.

Figure 6

Macroscopic images with vessels per unit area and histochemically stained images with thickness of the vascular layer on the subcutaneously implanted alginate gels with FGF-2 with syndecan-4 (sdc-4) proteoliposomes. Alginate gels containing PBS, FGF-2 and sdc-4 proteoliposomes with FGF-2 were subcutaneously implanted on the back of mice. The gels were harvested after seven days and macroscopically imaged (A). Vascularity around the gel was determined for the macroscopic images using Metamorph (B). The gels were frozen, sectioned and H&E stained (C). The thickness of the vascular layer was quantified by analyzing the H&E stained images on Metamorph (D). The sections were immunostained with anti-PECAM antibody and imaged with an epifluorescence microscope (E). Panel A - size bar = 3mm. Mag. size bar = 1mm. Panel C - size bar = 1mm. Mag. size bar = 250μm. Panel E - size bar = 250μm. *Statistically different from Ob/Ob group (p < 0.05). n=5