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A, Nested PCR at the tpi locus for identification of Giardia assemblages (Lane 1 and 5—molecular weight marker; Lane 2—assemblage A; Lane 3—assemblage B; Lane 4—coinfection of assemblage A and B). B, TpiA RFLP with RsaI for assemblage A (Lane 1 and 4—molecular weight marker; Lane 2—assemblage A–I; Lane 3—assemblage A–II).
