Figure 4. IL-7 and BTLA form a negative feedback loop.

Equal numbers of lymphocytes from the iLN of Btla^+/+ and Btla^−/− mice were cultured for four days with or without 10 ng/ml IL-7.

(A) Expression of CD27 and Vγ2 with or without IL-7 in TCRγδ⁺TCRβ⁻ gated cells.

(B) Percentage (%) of Btla^+/+ and Btla^−/− CD27⁻ γδ T cells with or without IL-7.

(C–F) The fold difference in γδ T cell cellularity as defined by the ratio of cell number with or without IL-7. (C) Vγ2⁻CD27⁻ γδ T cells, (D) Vγ2⁺CD27⁻ γδ T cells, (E) Vγ2⁻CD27⁺ γδ T cells, (F) Vγ2⁺CD27⁺ γδ T cells (FACS plots are representative of four experiments; in graphs each symbol represents an experiment and lines are medians).

(G–K) Lymphocytes from the iLN and PP of Rorc^gfp/+ mice were enriched for ILCs and CD27⁻ γδ T cells and cultured with or without IL-7 for two days (cells are RORγt⁺TCRβ⁻ gated). (G) Expression of BTLA and RORγt in iLN γδ T cells (top) and PP ILCs (bottom) with or without IL-7. (H) Numbers of BTLA⁺ iLN γδ T cells. (I) MFI for BTLA expression in iLN γδ T cells. (J) Numbers of BTLA⁺ PP ILCs. (K) MFI for BTLA expression in PP ILCs. FACS plots are representative of seven (iLN) or five (PP) independent experiments. In graphs each symbol represents an experiment and lines are medians. See also Figure S4.