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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Chem Biol. 2013 Dec 12;20(12):1536–1546. doi: 10.1016/j.chembiol.2013.11.005

Figure 5.

Figure 5

BHQ-O-5HT acts on mouse DRG neurons in culture. (a) Brightfield image of mouse DRG neurons in culture showing placement of the field recording pipette (black arrowhead) and direction toward the microinjection pipette (open white arrowhead), which was located 100 μm from the cells and is out of the field of view. (b) Pressure injection of 1 nL of a 100 μM buffered 5-HT solution induced activity in the medium sized DRG neuron shown in (a) but no change in activity was observed upon exposure to a 1-ms pulse of 365-nm light (1PE). (c) Although pressure ejection of a 1-mM buffered BHQ-O-5HT solution induced a small change baseline activity in the same neuron as (b), a significant change in activity was observed after exposure to a 1-ms pulse of 365-nm light. The traces comparing 5-HT to BHQ-O-5HT are temporally aligned to facilitate comparison.