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. 2014 Jan 5;5(2):115–124. doi: 10.7150/jca.8084

Figure 2.

Figure 2

Assay of PAI-1 concentration standards by colorimetric ELISA and ILPCR. A) Colorimetric ELISA using the Sekisui Diagnostics ELISA assay kit performed by following the manufacturer's instructions. B) ILPCR assay performed suing PAI-1 standards from the ELISA assay, but with additional serial dilutions of 100, 10, and 1 pg/mL. The Streptavidin-HRP conjugate with colorimetric development was replaced with NeutrAvidin plus detection liposomes with qPCR quantification. Data points (black dots), standard deviations (blue bars), regression analysis (solid orange plot lines), ILPCR assay blank (solid red line), IPCR assay threshold (magenta dotted line).