Table 1.

Recovery of protein from frozen human breast tumor tissue

Parameter	Standard Methoda	Pressure-Cycling Methodb
Total Protein Recoveredc	2.8 ± 0.22 mg/mL	3.0 ± 0.27 mg/mL
PAI-1 Recovered	68 ± 7.4 ng/mL	75 ± 6.8 ng/mL
Normalized PAI-1 Recovery	24 ± 3.3 ng/mg	25 ± 3.2 ng/mg
Total Processing Time	18 hr	2.3 hr

^aThe standard method used overnight stirring of the homogenate as described in the text. ^bThe pressure-assisted method consists of high-pressure cycling on a commercial Barocycler NEP2320 using FT500 pulse tubes as described in the text. ^cTotal protein was measured using the BCA colorimetric protein assay.