Fig. 1.

GPVI-specific ROS production does not involve Nox2. (A) Washed platelets from wildtype (Wt) or Nox2-knockout (KO) mice were pre-loaded with 10 µM H₂DCFDA, then stimulated with 1 µg/mL CRP for 2 min and monitored for ROS production by flow cytometry. Data are mean±SEM, n=8, non-significant (ns). (B) H₂DCFDA loaded neutrophils from Wt or Nox2-KO mice were stimulated with 5 µM PMA for 5 min and analyzed by flow cytometer to assess ROS production. Data are mean±SEM, n=4, ^⁎⁎⁎P<0.001. Two-way ANOVA with Bonferroni post-hoc analysis were performed for A and B.