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. 2014 Jan 15;28(2):121–126. doi: 10.1101/gad.230599.113

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© 2014 Bahmanyar et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 2. — Mitotic nuclei in cnep-1Δ mutants are wrapped in an additional ER layer. (A,B) Transmission electron micrographs of the nuclear regions of prophase (top) and prometaphase/metaphase (bottom) high-pressure-frozen embryos (eight to 16 cells) at the indicated mitotic stages (n = 13 mitotic cells for control; n = 11 mitotic cells for cnep-1Δ). Magnified images of boxed regions are paired with micrographs pseudocolored to highlight the nuclear envelope/ER lumens (red) that separate the cytoplasm (yellow) and nucleus (blue). Cinched-in regions along the nuclear envelope in A are where nuclear pore complexes are inserted. Arrows (dark red) point to isolated regions where two adjacent lumens are detected. One-hundred percent of cnep-1Δ nuclear envelopes during late stages of NEBD (n = 7), as compared with none in control (n = 6), displayed an additional double-membrane sheet encircling the nuclear envelope. Bars, 1 μm.