Table 3.
Genes coding for ion channels and transporters involved in CSF and AH production in the CPE and NPE
| Ion channels and transporters | Present in | Coding genes | High gene expr. | Sign. diff. gene expr. |
|---|---|---|---|---|
| Na+/K+ ATPase |
CPE and CBE |
ATP1A1 |
Both |
NPE |
|
ATP1A2 |
Both |
|
||
|
ATP1A3 |
CPE |
|
||
|
ATP1A4 |
|
|
||
|
ATP1B1 |
Both |
CPE |
||
|
ATP1B2 |
Both |
|
||
|
ATP1B3 |
Both |
NPE |
||
| Na+/2Cl-/K+ co-transport |
CPE and CBE |
SLC12A1 |
|
|
|
SLC12A2 |
CPE |
CPE |
||
| Na+/Cl- symport |
CBE |
SLC12A3 |
|
|
| K+/Cl- symport |
CPE |
SLC12A4 |
|
|
|
SLC12A5 |
|
|
||
|
SLC12A6 |
|
|
||
|
SLC12A7 |
Both |
|
||
|
SLC12A9 |
|
|
||
| Cl-/HCO3– exchanger |
CPE and CBE |
SLC4A1 |
|
|
|
SLC4A2 |
|
CPE |
||
|
SLC4A3 |
|
|
||
| Na+/HCO3– symport |
CPE |
SLC4A4 |
NPE |
NPE |
|
SLC4A5 |
|
|
||
|
SLC4A7 |
|
|
||
|
SLC4A8 |
|
|
||
|
SLC4A9 |
|
|
||
|
SLC4A10 |
CPE |
CPE |
||
| Na+/H+ exchanger |
CPE and CBE |
SLC9A1 |
|
|
|
SLC9A2 |
|
|
||
|
SLC9A3 |
|
|
||
|
SLC9A4 |
|
|
||
|
SLC9A5 |
|
|
||
|
SLC9A6 |
|
|
||
| K+ channel |
CPE and CBE |
KCNA4 |
|
NPE |
|
KCNA5 |
CPE |
CPE |
||
|
KCNAB1 |
NPE |
|
||
|
KCNB2 |
|
NPE |
||
|
KCNE1 |
|
CPE |
||
|
KCNF1 |
|
CPE |
||
|
KCNG1 |
|
NPE |
||
|
KCNH2 |
|
CPE |
||
|
KCNJ13 |
Both |
|
||
|
KCNK1 |
CPE |
CPE |
||
|
KCNN2 |
|
CPE |
||
|
KCNN3 |
|
CPE |
||
|
KCNS1 |
|
NPE |
||
| Cl- channel |
CPE and CBE |
CLCN1 |
|
|
|
CLCN2 |
|
|
||
|
CLCN3 |
|
|
||
|
CLCN4 |
|
|
||
|
CLCN5 |
|
|
||
|
CLCN6 |
|
|
||
|
CLCN7 |
|
|
||
| Na+ channel |
CPE and CBE |
SCN1A |
|
|
|
SCN1B |
|
|
||
|
SCN2A |
|
CPE |
||
|
SCN2B |
|
NPE |
||
|
SCN3A |
|
|
||
|
SCN3B |
|
NPE |
||
|
SCN4A |
|
|
||
|
SCN4B |
NPE |
NPE |
||
|
SCN5A |
|
|
||
|
SCN7A |
|
|
||
|
SCN8A |
|
|
||
|
SCN9A |
|
|
||
|
SCN10A |
|
|
||
|
SCN11A |
|
|
||
| Water channel |
CPE and CBE |
AQP1 |
Both |
|
| |
|
AQP2 |
|
|
| |
|
AQP3 |
|
|
| |
|
AQP4 |
|
CPE |
| |
|
AQP5 |
|
|
| |
|
AQP7 |
|
|
| |
|
AQP8 |
|
|
| |
|
AQP9 |
|
|
| |
|
AQP10 |
|
|
| AQP11 |
This table lists the ion channels and ion transporters involved in cerebrospinal fluid (CSF)/aqueous humor (AH) production, their presence in choroid plexus epithelium (CPE) and/or non-pigmented epithelium (NPE) of the ciliary body epithelium according to literature [20,30] and the gene expression profiles in the CPE and NPE according to our microarray gene expression studies. Column 1: Ion channels and transporters involved in CSF/AH production. For the K+ channel, we only presented the highly and/or statistical significant different expressed genes of the CPE and NPE; Column 2 - Present in: The presence of the different ion channels and ion transporters are reviewed in [30] for the CPE and [20] for the NPE; Column 3 - Coding genes: the genes coding for the ion channels and ion transporters listed in column 1; Column 4 - High gene expr.: Here we indicate the genes that are highly expressed in the CPE and/or NPE according to our gene expression microarray data of the CPE and NPE. Highly expressed gene are genes with mean expression values >90th percentile. This means that these genes have an expression intensity that falls into the highest 10% intensity values of the microarray. Both = high expression in both CPE and NPE; Column 5 - Sign. diff. gene expr.: Here we indicate the genes that were statistically significant higher expressed in the CPE or NPE compared with the NPE and CPE, respectively, according to our gene expression microarray data of the CPE and NPE. The selection criteria were fold change > 5 and p-value < 0.001.