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. Author manuscript; available in PMC: 2014 Feb 3.
Published in final edited form as: Nat Chem Biol. 2010 Jun 27;6(8):621–629. doi: 10.1038/nchembio.400

Figure 5. Effect of physiologic stimuli or effect of combining physiologic stimuli with addition of agRNAs on expression of PR mRNA, the 3’ noncoding RNA, and the 5’ noncoding RNA.

Figure 5

qPCR analysis showing the effect of physiologic stimuli on transcript expression in (a) T47D cells and (b) MCF7 cells. For the sample labeled “serum stripped followed by full media” cells were grown in serum stripped media. The media was replaced by full media for one day prior to harvesting. qPCR analysis of the effect of physiologic stimuli and agRNA (25 nM) addition on transcript expression in (c) T47D cells and (d) MCF7 cells. PR 3’NCR: 3’ noncoding PR RNA. PR 5’NCR: 5’ noncoding PR RNA. SS: Serum-stripped media. FM: full media. E2: 17ß estradiol treatment (100 nM). IL1ß: interleukin 1ß treatment (10 ng/mL). EGF: epidermal growth factor treatment (100 ng/mL). Results are from 3-6 independent replicates.