Figure 3. Progenitors in Runx1^{HTY350-352AAA} bone marrow.

(A) Bone marrow cells from wildtype or Runx1^{HTY350-352AAA} animals were stained using antibodies to c-Kit, Sca1, CD16/32, CD34, IL7Ralpha, AA4.1 and Lineage markers (CD3, CD11b, B220, Ter119, Gr1) and analyzed by flow cytometry. Lineage negative cells were gated and are plotted in panel A to highlight the Lin-Sca1+Kit+ (LSK), myeloid potential L-S-K+ and lymphoid potential L-S+K− fractions. (B) Quantification of multiple experiments performed as depicted in A, normalizing to the wildtype average of each experiment (n=13 WT, 13 HTY). (C) Lineage negative and IL7R alpha positive bone marrow cells were gated and c-Kit versus AA4.1 was plotted to measure CLPs. (D) Quantification of multiple experiments in C (n=6 WT, 6 HTY). (E) Lineage negative, c-Kit positive, Sca1 negative (L-S-K+) cells from panel A were plotted as CD16/32 versus CD34 to measure the CMP, GMP and MEP progenitor populations. (F) Quantification of multiple experiments as in E, normalizing to the wildtype average of each experiment (n=10 WT, 10 HTY). * p<0.05, calculated by Student’s T test. All error bars are SEM.