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Dose titration of splice-correcting plasmid and micro-minicircle (miMC) in vitro in HeLaLuc705 cells 24 hours after transfection. (a) Expression of splice-correcting 705 snRNA, as determined by qPCR, equilibrated against the endogenous control RNU24 and normalized to expression from 1.6 µg plasmid (data shown as mean + 95% confidence interval, n = 2). (b) Splice-corrected luciferase mRNA assayed by two step RT-PCR (data shown as mean + SEM, n = 2).
