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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Nature. 2013 Aug 25;501(7467):444–448. doi: 10.1038/nature12487

Figure 4. PAS-cap interaction with the CNBHD, and predicted tetramer of the eag domain-CNBHD complex.

Figure 4

a, Cartoon representation of the PAS-cap region. The PAS domain and the CNBHD are shown as surface (green and blue, respectively) with the PAS-cap regions shown as cartoon and position E627 as orange sticks. b, Alignment of the eag domain (green) with representative hERG1 eag domain NMR structures. The X-ray structures of the eag domain-CNBHD complex of mEAG1 and the isolated eag-domain of hERG1 (PDB code 4HP9) are shown as green and orange cartoons, respectively, and the NMR structures are in yellow (PDB code 2L0W), cyan (PDB code 2L4R), and purple (PDB code 2L1M). The PAS domains are represented as grey ribbons. c, Normalized conductance plotted as a function of the test voltages for mEAG1 wild-type and mutant channels (error bars indicate SEM, n=3-68 cells, P < 0.001, Student's t-test). d, Alignment of the eag domain-CNBHD complex with the CNBD of mHCN2 (PDB 1Q5O), color coded as in Fig. 1b.