Table 1.
Sense and antisense primers used for reverse transcription-polymerase chain reaction (RT-PCR).
| Target mRNA | Primer sequences | |
|---|---|---|
| GADPH | Sense | 5′-GAAGGTGAAGGTCGGAGTC-3′ |
| Antisense | 5′-GAAGATGGTGATGGGATTTC-3′ | |
|
| ||
| IL-4 | Sense | 5′-ACCTTGCTGTCACCCTGTTC-3′ |
| Antisense | 5′-GTTGTGAGCGTGGACTCATTC-3′ | |
|
| ||
| IL-10 | Sense | 5′- GCTATGTTGCCTGCTCTT-3′ |
| Antisense | 5′- CCAAGTAACCCTTAAAGTCC-3′ | |
|
| ||
| IFN-γ | Sense | 5′-ACTCATTGAAAGCCTAGAAAGTC-3′ |
| Antisense | 5′-TCTTCTTATTGGCACACTCTCTA-3′ | |
|
| ||
| FVII | Sense | 5′-ACTCACGGACTGCTGCTT-3′ |
| Antisense | 5′- CGCCATCGCTGTAAATAG-3′ | |
GADPH: glyceraldehydes-3-phosphate dehydrogenase; IFN-γ: interferon-γ; IL: interleukin.