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. 2013 Jun 3;15(10):2841–2849. doi: 10.1111/1462-2920.12153

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© 2013 The Authors. Environmental Microbiology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution–NonCommercial–NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Verification of cell recovery rate using the bilayer cell separation method.A. Percent recovery of cells from control sediment A (Site C9001, Core 10H-1), B (Site C0008A, Core 7H-2) and C (Site U1365C, core 5H-1) mixed with cultured Escherichia coli cells. Varying amounts (2, 4, 8 × 10⁻³ cm³) of sediment were utilized in the separation.B. Percent recovery of cells from natural sediment samples. The number of microbial cells in 10 samples from CK06-06, 5 samples from IODP Expedition 316 and 10 samples from IODP Expedition 329 was determined without and with bilayer separation, and the average percent recovery for each sample location is shown. Bars show the maximum and minimum percentage of recovery.