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. 2013 Jun 3;15(10):2841–2849. doi: 10.1111/1462-2920.12153

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© 2013 The Authors. Environmental Microbiology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution–NonCommercial–NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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FCM analysis of density-separated sediment samples.A. FCM cytogram (scatter plot) of the sample from Site C9001, Core 1H-1 following separation and staining with SYBR Green I. Lower right portion below the solid line shows the region of cell-derived fluorescence signals. Signals surrounded by a solid circle are from volume calibration beads.B. Number of microbial cells as determined by microscopic counting and FCM. Dotted line shows the 1:1 line for the counts determined by microscopy and FCM.