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DNA amplification from Dermacentor nitens by nested-PCR targeting the B. burgdorferi s.l. 5S (rrf) - 23S (rrl) intergenic spacer region. Lane 1: Tick sample Jataí 01- (D. nitens); lanes 2 and 3: negative controls (extraction and nested-PCR amplification); lane 4: positive control with PBi strain of B. garinii); M - DNA marker (100 bp).
