Table 3.
COMT methylation in healthy human subjects versus individual factors
|
CpG Units |
Age | Sex a | AUDIT score | Ethnicity b | SES | COMT Met alleles |
|---|---|---|---|---|---|---|
| r (95% CI) p | ||||||
| 001_1 |
-.099 (-.401, .390) .565 |
.071 (-.397, .357) .681 |
-.021 (-.461, .381) .905 |
-.124 (-.556, .247) .470 |
.039 (-.236, .262) .821 |
.003 (-.381, .474) .988 |
| 001_3.4 |
-.022 (-.241, .292) .901 |
-.051 (-.331, .313) .766 |
-.085 (-.411, .336) .612 |
-.187 (-.454, .216) .274 |
-.137 (-.546, .164) .425 |
-.084 (-.322, .331) .624 |
|
001_34 |
.037 (-.221, .355) .830 |
-.081 (-.352, .243) .639 |
-.206 (-.543, .110) .227 |
-.201 (-.568, .166) .239 |
-.331 (-.653, .044) .049* |
-.018 (-.290, .336) .919 |
| 001_avg |
-.029 (-.256, .265) .865 |
-.034 (-.348, .223) .845 |
-.169 (-.482, .158) .323 |
-.235 (-.520, .146) .168 |
-.232 (-.521, .065) .174 |
-.037 (-.358, .343) .830 |
| 002_1 |
-.051 (-.373, .284) .768 |
.058 (-.258, .415) .737 |
.038 (-.263, .386) .825 |
.195 (-.134, .528) .256 |
-.189 (-.479, .124) .269 |
.130 (-.214, .454) .451 |
| 002_5 |
.073 (-.382, .440) .673 |
.168 (-.161, .529) .326 |
.038 (-.270, .338) .826 |
-.066 (-.386, .311) .701 |
-.235 (-.542, .117) .168 |
.282 (-.090, .600) .096 |
| 002_10 |
.002 (-.348, .382) .991 |
.081 (-.222, .434) .638 |
-.044 (-.351, .278) .797 |
.145 (-.208, .498) 398 |
-.222 (-454, .046) .194 |
.257 (-.049, .514) .130 |
| 002_avg |
-.009 (-.336, .338) .960 |
.097 (-.195, .463) .574 |
.009 (-.307, .324) .959 |
.140 (-.208, .494) 415 |
-.230 (-.470, .092) .177 |
.227 (-.100, .495) .184 |
|
003_1 |
-.118 (-.461, .229) .479 |
-.199 (-.484, .137) .231 |
-.052 (-.334, .230) .758 |
.421 (.103, .696) .008** |
-.081 (-260, .412) .665 |
.138(-.182, .423) .408 |
| 003_3 |
-.154 (-.415, .131) .363 |
.165 (-.152, .467) .329 |
-.099 (-.379, .222) .561 |
-.278 (-.520, .008) .096 |
.074 (-.260, .412) .665 |
-.013 (-.376, .308) .940 |
| 003_6 |
.135 (-.128, .387) .433 |
-.188 (-.453, .120) .272 |
.151 (-.146, .443) .379 |
-.162 (-.396, .112) .344 |
-.223 (-.435, .046) .190 |
-.092 (-.450, .291) .593 |
| 003_avg |
.031 (-.301, .306) .853 |
-.107 (-.420, .182) .522 |
.061 (-.252, .339) .717 |
-.049 (-.390, .311) .769 |
-.187 (-.509, .209) .260 |
-.013 (-.359, .352) .937 |
|
005_2 |
-.239 (-.555, .214) .148 |
.028 (-.327, .356) .866 |
-.159 (-.434, .156) .341 |
-.209 (-.503, .184) .208 |
.041 (-.261, .366) .806 |
-.333 (-.596, -.063) .041* |
| 005_3 |
-.065 (-.343, .273) .700 |
-.125 (-.422, .201) .454 |
-.116 ( -.391, .203) .489 |
-.159 (-.447, .176) .340 |
-.091 (-.358, .209) .587 |
-.193 (-.513, .128) .247 |
| 005_5 |
-.127 (-.416, .236) .446 |
.039 (-.283, .378) .815 |
-.068 (-.401, .262) .685 |
-.144 (-.438, .197) .388 |
-.136 (-.398, .202) .414 |
-.317 (-.592, -.047) .053 |
| 005_7 |
-.147 (-.451, .229) .380 |
-.120 (-.403, .216) .922 |
-.072 (-.354, .229) 668 |
-.176 (-.444, .184) .291 |
-.099 (-.400, .216) .553 |
.265 (-.547, .039) .107 |
|
005_8 |
-.103 (-.407, .280) .540 |
-.016 (-.349, .327) .922 |
-.120 (-.405, .192) .474 |
-.234 (-.523, .142) .157 |
-.074 (-.351, .264) .657 |
-.359 (-.601, -.072) .027* |
| 005_9 |
-.058 (-.405, .381) .731 |
-.029 (-.342, .324) .863 |
-.207 (-.448, .117) .213 |
-.196 (-.511, .146) .239 |
.018 (-.329, .332) .914 |
-.077 (-.374, .160) .646 |
|
005_10 |
-.059 (-.381, .298) .723 |
-.064 (-.388, .276) .701 |
-.103 (-.416, .238) .539 |
-.276 (-.535, .084) .094 |
.120 (-.177, .431) .472 |
-.403 (-.632, -.108) .012* |
|
005_17 |
.038 (-.230, .336) .819 |
-.193 (-.496, .172) .245 |
-.144 (-.418, .135) .389 |
-.290 (-.551, .030) .078 |
.086 (-.241, .413) .607 |
-.383 (-.613, -.137) .018* |
|
005_18 |
-.022 (-.310, .327) .897 |
-.181 (-.471, .153) .284 |
-.203 (-.478, .131) .229 |
-.345 (-.623, -.026) .037* |
.007 (-.297, .305) .968 |
-.379 (-.585, -.123) .021* |
|
005_avg |
-.098 (-.414, .299) .558 |
-.083 (-.398, .234) .621 |
-.161 (-.435, .152) .333 |
-.257 (-.542, .099) .119 |
-.017 (-.358, .336) .921 |
-.325 (-.588, -.049) .046* |
| 006_3c |
-.031 (-.357, .241) .856 |
-.037 (-.364, .320) .823 |
-.056 (-.347, .256) .738 |
-.336 (-.644, .051) .039* |
.158 (-.200, .480) .342 |
.013 (-.333, .309) .937 |
| 006_4 |
-.027 (-.333, .259) .873 |
-.099 (-.412, .263) .554 |
-.094 (-.242, .318) .774 |
-.242 (-.529, .131) .143 |
.152 (-.166, .567) .363 |
-.093 (-.410, .225) .580 |
| 006_7 |
-.060 (-.365, .221) .722 |
.079 (-.241, .392) .638 |
.048 (-.242, .318) .774 |
-.179 (-.433, .165) .282 |
.122 (-.144, .361) .467 |
-.198 (-.514, .119) .232 |
|
006_8 |
-.078 (-.390, .236) .642 |
-.096 (-.399, .240) .566 |
-.043 (-.402, .311) .798 |
-.102 (-.508, .271) .542 |
.213 (-.100, .479) .200 |
-.932 (-.970, -.879) < .001***
|
| 006_9 |
-.052 (-.354, .264) .758 |
-.080 (-.399, .292) .635 |
-.047 (-.338, .278) .782 |
-.211 (-.489, .129) .204 |
.036 (-.243, .343) .829 |
-.306 (-.571, -.040) .061 |
| 006_10 |
.037 (-.248, .331) .825 |
-.065 (-.415, .258) .697 |
-.095 (-.392, .254) .570 |
-.201 (-.521, .162) .226 |
.127 (-.147, .405) .449 |
-.178 (-.471, .115) .285 |
| 006_12 |
-.046 (-.338, .311) .782 |
-.093 (-.410, .267) .579 |
-.046 (-.344, .300) .782 |
-.199 (-.467, .181) .231 |
.058 (-.263, .356) .731 |
-.295 (-.557, -.041) .072 |
| 006_13 |
-.077 (-.383, .255) .645 |
-.027 (-.354, .309) .874 |
.007 (-.315, .371) .968 |
-.112 (-.470, .267) .504 |
.072 (-.164, .339) .669 |
-.313 (-.590, -.021) .056 |
|
006_14.15 |
-.054 (-.359, .239) .746 |
-.109 (-.455, 236) .515 |
-.059 (-.381, .319) .723 |
-.188 (-.481, .175) .259 |
.120 (-.162, .407) .474 |
-.329 (-.579, -.063) .044* |
|
006_16 |
-.050 (-.362, .278) .766 |
-.128 (-.450, .221) .445 |
-.019 (-.338, .308) .911 |
-.207 (-.494, .182) .212 |
.086 (-.222, .396) .609 |
-.337 (-.600, -.062) .039* |
|
006_avg |
-.048 (-.350, .270) .776 |
-.065 (-.403, .266) .697 |
-.040 (-.348, .276) .813 |
-.212 (-.531, .161) .201 |
.120 (-.175, .395) .474 |
-.411 (-.638, -.163) .010* |
|
008_3 |
-.109 (-.370, .195) .528 |
-.253 (-.557, .125) .136 |
-.033 (-.269, .221) .850 |
-.160 (-.482, .206) .351 |
.168 (-.194, .469) .326 |
.557 (.278, .738) < .001***
|
| 008_4 |
.199 (-.053, .411) .238 |
.056 (-.368, .275) .742 |
-.086 (-.350, .401) .613 |
-.203 (-.412, .097) .229 |
-.027 (-.247, .198) .875 |
.103 (-.484, .381) .543 |
| 008_6 |
.144 (-.175, .413) .394 |
.110 (-.240, .443) .516 |
.082 (-.210, .429) .631 |
-.189 (-.471, .151) .262 |
.007 (-.275, .322) .968 |
-.009 (-.340, .297) .956 |
| 008_10 |
.110 (-.169, .397) .516 |
.088 (-.256, .389) .606 |
.054 (-.300, .343) .751 |
-.205 (-.566, .186) .223 |
-.008 (-.347, .314) .965 |
-.196 (-.519, .101) .245 |
|
008_avg |
.078 (-.219, .352) .647 |
-.061 (-.375, .260) .718 |
-.011 (-.253, .293) .946 |
-.257 (-.578, .146) .125 |
.081 (-.197, .358) .632 |
.330 (-.035, .562) .046* |
| 010_2 |
-.050 (-.390, .293) .765 |
.017 (-.351, .325) .919 |
-.170 (-.475, .154) .308 |
-.282 (-.595, .145) .086 |
.106 (-.228, .410) .527 |
-.228 (-.521, .043) .169 |
|
010_3.4 |
-.140 (-.41, .193) .401 |
-.021 (-.331, .301) .901 |
-.085 (-.411, .336) .612 |
-.202 (-.492, .196) .224 |
.094 (-.244, .385) .575 |
-.362 (-.605, -.073) .026* |
| 010_6 |
-.154 (-.438, .207) .357 |
-.010 (-.352, .324) .954 |
-.146 (-.469, .173) .383 |
-.188 (-.506, .221) .259 |
.112 (-.223, .385) .502 |
-.317 (-.591, -.004) .053 |
|
010_8 |
-.159 (-.434, .166) .339 |
-.048 (-.381, .271) .775 |
-.194 (-.502, .171) .243 |
-.174 (-.494, .219) .295 |
.117 (-.229, .413) .482 |
-.336 (-.599, -.052) .039* |
| 010_14d |
-.145 (-.414, .177) .386 |
.004 (-.373, .325) .980 |
-.168 (-.483, .180) .313 |
-.218 (-.501, .156) .189 |
.040 (-.291, .414) .814 |
.241 (-.142, .546) .145 |
| 010_ avg | -.128 (-.432, .230) .444 | -.015 (-.342, .296) .928 | -.154 (-.495, .192) .356 | -.220 (-.555, .182) .185 | .110 (-.238, .395) .509 | -.318 (-.587, -.031) .052 |
Pearson partial correlation coefficients were calculated using a bootstrapping procedure based on percent methylation values for COMT CpG units within the top 40th percentile for differential methylation within the healthy human sample. Correlations in each column controlled for all other column variables. Results are based on n = 48 samples. AUDIT, Alcohol Use Disorders Identification Test; CI, confidence interval; SES, socioeconomic status. amale = 0, female = 1; bwhite = 1, non-white = 0 cNot within 40th percentile for differential methylation, but selected a priori based on [56]. dNot within 40th percentile for differential methylation, but selected a priori because CpG unit is located within the rs4680 SNP and present only in COMTVal allele [33]. *p < .05, **p < .01, ***p < .001, uncorrected for FWE, FDR corrected (p < .05) .