Figure 2.
Comparative cellular activities of L- and D-polyQ versions of PKKKRKVGGQ25KK amyloid fibrils. (a) Percent of WT PC12 cells undergoing lytic death (LDH release) after 24 hrs exposure to different concentrations of L- or D-polyQ versions of PKKKRKVGGQ25KK amyloid; (b) percent of Htt-exon1-Q25-EGFP cells treated with 2.5 μM (monomer equivalent) of PKKKRKVGGQ25Ccy5KK amyloid that develop green puncta (see Figure 3) at different growth times. Values in a and b are from three independent experiments and are expressed as mean ± SEM (**, p < 0.01, ***, p < 0.001).