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CD of apo-ZF4 (free), Zn-ZF4 and Zn-ZF4 + 1 at 25 ºC. Apo-ZF4 is characterized by a minimum at 198 nm expected of a random coil. Zn-ZF4 exhibits a maximum at 190 nm, a minimum at 208 nm, and a shoulder at 224 nm, characteristic of a ββα motif. 1 addition to Zn-ZF4 disrupts the ββα motif as illustrated by a loss of the 208 minimum of the ββα motif and a shift towards the random coil 198 nm minimum.
