Figure 3. Induction of partial demethylation and active chromatin modifications.

A) Ectopic expression of miR-34b induces partial demethylation of CpG islands. B) Enrichment of active chromatin modifications by overexpression of miR-34b. ChIP assay in PC3 cells transfected with miR-34b or cont-miR was performed. Quantification of the immunoprecipitated upstream region of miR-34b was determined by quantitiative real-time PCR and normalized to input DNA. Signals were also confirmed by conventional PCR and PCR product for each sample was resolved on 2% agarose gel and visualized by staining with ethidium bromide.