FIGURE 2:

KCa3.1 blocker TRAM34 prevented TGF-β1-induced PAI-1, MMP2 and MMP9 expression in human renal interstitial fibroblasts. Human renal interstitial fibroblasts were treated with control, TGF-β1 (2 ng/mL) or TGF-β1 (2 ng/mL) combined with TRAM34 (2 μM) for 48 h. Quantitative RT–PCR and western blot results demonstrated TRAM34 suppressed TGF-β1-induced PAI-1 mRNA (A) and protein expression (B) as well as MMP2 (C) and MMP9 (D) mRNA expression in cultured human renal interstitial fibroblasts. (E) Zymographic analysis showed TRAM34 reversed TGF-β1-induced proteolytic activity of MMP2 and MMP9 in human renal interstitial fibroblasts. Results are presented as means ± SEM. *P < 0.05 and **P < 0.01, n = 3.