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. 2013 Oct 28;29(2):313–324. doi: 10.1093/ndt/gft431

Table 1.

Nucleotide sequences of the primers used for qRT–PCR

Species Molecules Forward primer (5′–3′) Reverse primer (5′–3′)
Human α-SMA CCGACCGAATGCAGAAGGA ACAGAGTATTTGCGCTCCG
COL1A TCTGGAGAGGCTGGTACTGC GAGCACCAAGAAGACCCTGA
COL4A1 CGGGTACCCAGGACTCATAG GGACCTGCTTCACCCTTTTC
FN GCGAGAGTGCCCCTACTACA GTTGGTGAATCGCAGGTCA
FSP-1 CTTGCACACGCTGTTGCTAT CGAGTACTTGTGGAAGGTGGA
GAPDH AGCCACATCGCTCAGACAC GCCCAATACGACCAAATCC
MMP2 ATAACCTGGATGCCGTCGT AGGCACCCTTGAAGAAGTAGC
MMP9 GAACCAATCTCACCGACAGG GCCACCCGAGTGTAACCATA
PAI-1 TCCAGCAGCTGAATTCCTG GCTGGAGACATCTGCATCCT
Vimentin AGATGGCCCTTGACATTGAG TGGGTATCAACCAGAGGGAG
Mouse α-SMA ACTCTCTTCCAGCCATCTTTCA ATAGGTGGTTTCGTGGATGC
β-actin CAGCTGAGAGGGAAATCGTG CGTTGCCAATAGTGATGACC
COL1A CATGTTCAGCTTTGTGGACCT GCAGCTGACTTCAGGGATGT
FN CGGAGAGAGTGCCCCTACTA CGATATTGGTGAATCGCAGA
FSP-1 GGAGCTGCCTAGCTTCCTG TCCTGGAAGTCAACTTCATTGTC
MMP2 TAACCTGGATGCCGTCGT TTCAGGTAATAAGCACCCTTGAA
MMP9 ACGACATAGACGGCATCCA GCTGTGGTTCAGTTGTGGTG
Vimentin CCAACCTTTTCTTCCCTGAA TGAGTGGGTGTCAACCAGAG