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. Author manuscript; available in PMC: 2014 Feb 3.
Published in final edited form as: J Biol Chem. 2005 Oct 19;280(52):42655–42668. doi: 10.1074/jbc.M505910200

Figure 2. Characterization of the K08E3.7 KO transgenic strain.

Figure 2

(A) A life span curve shows that K08E3.7 KO (square) strain has life span that is 15.4% shorter than the non-tg (diamond), wild type (WT, triangle) and A53T (circle) α-synuclein expressing strains. Mean life span for the non-tg, WT and A53T α-synuclein expressing strains was 18.5 ± 0.169, while the mean life span of the K08E3.7 KO strain was 15.65 ± 0.211. p<0.01 compared to the non-tg. (B) Immunoblot probed with monoclonal ubiquitin antibody (upper panel) or actin (lower panel, loading control) showing higher levels of high molecular weight ubiquitin conjugates present in the non-tg (lane 1) strain than the K08E3.7 knockout (KO, lane 2) strain. (C) Longer exposure of immunoblot shows high molecular weight smears of ubiquitin conjugates in the stacking gel in the lysates from the non-tg strain. (D) Densitometric quantification of immunoblot from panel B blot; (*) p<0.01.