Table 1.
Sequence of the external and internal primers for nested qRT-PCR on mRNA from laser captured serotonin neurons.
| Synaptic Plasticity Primers | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Sequence | TM | Amplicon size | Name | Sequence | TM | Amplicon size | ||
| RhoA | Profilin | ||||||||
| ExtF | TGGGGCAGGAAGATTATGATCG | 65.3 | 436 | ExtF | GGCGCTGCGGTAAGGAG | 63.05 | 305 | ||
| ExtR | GCAGCTCTCGTAGCCATTTCA | 62.85 | ExtR | AGCTCTGCCGACAGCAACAT | 63.44 | ||||
| IntF | CCCTCTCCTACCCAGATACCG | 62.08 | 251 | IntF | CCGCCGCTGGTTAGTCAG | 62.9 | 251 | ||
| IntR | CCCAAAAAGCACCAATCCTGTT | 64.37 | IntR | CTCTACCAGCTCTGCCGACAG | 62.5 | ||||
| Rock2 | CDC42 | ||||||||
| ExtF | GGTTCGTCATAAGGCATCACA | 60.09 | 548 | ExtF | ACGACCGCTGAGTTATCCACA | 62.94 | 351 | ||
| ExtR | CCCCCACTAGCATCTCAAAAAG | 62.1 | ExtR | TTCTTCGGTTCTGGAGGCTC | 61.81 | ||||
| IntF | ATGCCTGGTGGAGACCTTGTA | 61.5 | 298 | IntF | TTTTCAGTGGTCTCTCCATCTTCAT | 62.4 | 202 | ||
| IntR | AGTAACCATCACCCCCTTGTG | 61.04 | IntR | GGTCACGGGCCAGCTTTT | 63.5 | ||||
| MLCK | IRSp53 | ||||||||
| ExtF | GCCACGTGAACCTGATCCA | 63.16 | 551 | ExtF | GGCTTTATCACCAGGATGTCACA | 63.3 | 463 | ||
| ExtR | CAGCAACCGGGAAACAAAGT | 62.34 | ExtR | CGGCTTTACCTTGAACTGAGGT | 61.85 | ||||
| IntF | GACCTCAAGCCGGAGAACATATT | 62.81 | 251 | IntF | TTTGGAAACATCTGTGGACTCTGTT | 63.2 | 263 | ||
| IntR | TCTGCATCTGTTTCCCCTAGAAAT | 62.4 | IntR | GGTGCACTCTCCTAGCATGCT | 61.9 | ||||
| CRIPAK | WAVE | ||||||||
| ExtF | CTCGCTTTGTGCCAATGTG | 61.43 | 1361 | ExtF | TTCAACTCCTCCCCCTCCA | 62.94 | 456 | ||
| ExtR | CCTCTCCTCTTCCATCTCATCC | 61.2 | ExtR | TTAGCTTCCTGTTCACGCTGC | 62.8 | ||||
| IntF | TCACACAAAGCCCTGGCAT | 62.6 | 154 | IntF | ACCTGGCATTCGACCATCAT | 62.6 | 253 | ||
| IntR | CCTACGTTATCTCGAGGTGCAG | 61.1 | IntR | ACGCTGCTCTTCTACTTTGCG | 62.09 | ||||
| LIMK2 | WASP | ||||||||
| ExtF | AGGTGGAGGATGCAATTAGCC | 62.5 | 356 | ExtF | GACAGAGCAGGCTGGGAGAA | 63.02 | 463 | ||
| ExtR | CAGGACCTCCCCATGGATTA | 62.01 | ExtR | TGCTATCAGGCTTTGAGCCC | 62.6 | ||||
| IntF | GTTGATTGAACATGACCCCGT | 61.9 | 251 | IntF | GGTCTGGGAGGACTGTTCCA | 62.47 | 208 | ||
| IntR | CAACGAAGGGATTCTGAGCG | 63.1 | IntR | GGTTCCTCTGAGGCTCTGGG | 63.56 | ||||
| Cofilin1 | ARP3 | ||||||||
| ExtF | GAAAAGCGCGCGAGATC | 63.5 | 451 | ExtF | TGCTAAGGCAGTAAAGGAGCG | 61.87 | 453 | ||
| ExtR | TGATGGCATCCTTGGAACTG | 62.02 | ExtR | CGCTGCATGTGGTGTGTAATG | 63.48 | ||||
| IntF | GGTGTGGCTGTCTCTGATGGT | 62.5 | 259 | IntF | ATGATACAGATGGGTCAAAATGGATTA | 62.7 | 252 | ||
| IntR | GCATCATAGAGGGCGTAGCG | 63.07 | IntR | TGAACATGGTTGAACCTCCAGA | 62.7 | ||||
| RAC1 | GAPDH | ||||||||
| ExtF | TTGTGGTAGATGGAAAACCGG | 61.9 | 351 | ExtF | CCCTCCAAAATCGAGTGTGGT | 60 | 315 | ||
| ExtR | TGTGAGCACCAAACACTCCAG | 62.3 | ExtR | AGGAAAACTGGCAGTGATGGC | 60 | ||||
| IntF | TGTCCGTGCAAAGTGGTATCC | 63.1 | 182 | IntF | CTGACACTGAGTACGTCGTGGA | 57 | 268 | ||
| IntR | TTTTGCAGCACCAATCTCCTT | 62.03 | IntR | TGGACTGTGGTCATGAGTCCTT | 58 | ||||
| PIP5 | PPIA | ||||||||
| ExtF | TTCAAGCTTTTGAAAGGCCTCAC | 62.93 | 405 | ExtF | TCGACGGCGAGCCCCT | 59 | 451 | ||
| ExtR | GCATTGTCATCCTGCACTTCC | 62.9 | ExtR | GTCCACAGTCAGCAATGGTGAT | 59 | ||||
| IntF | TTCCATCGCCGCCTTAAAAG | 63.3 | 201 | IntF | TCGTGCTCTGAGCACTGGAG | 59 | 301 | ||
| IntR | GAAATTGTGGTCGCCAAGGA | 63.25 | IntR | CATGCCTTCTTTCACTTTGCC | 58 | ||||
| Gelsolin | GUSP | ||||||||
| ExtF | GCCACCCTTCCTCTCCATCT | 62.8 | 454 | ExtF | TGATCGCTCACACCAAAGCTT | 59 | 453 | ||
| ExtR | CTGGAAAGGTAGGACAAGCCC | 62.2 | ExtR | TTTTGGTTGTCTCTGCCGAGT | 58 | ||||
| IntF | TGTGGCTGATGAGAACCCCT | 63 | 251 | IntF | ACTTCGGGCACCTGGAGTT | 58 | |||
| IntR | CCCGCCAGTTCTTGAAGAACT | 62.9 | IntR | TCATGAAATCGGCAAATTCC | 58 | 253 | |||