. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: Gastroenterology. 2013 Oct 2;146(1):245–256. doi: 10.1053/j.gastro.2013.09.050

Table 1.

Xenograft initiating capacity of FACS sorted AcTub^HI and ALDH^HI vs AcTub^LO/ALDH^LO cell populations.

Sorted Cell Population	No. Cells per injection	No. of tumors/No. of injections	TIC Frequency	P-value (vs. bulk)
Bulk	100	0/4	ND	ND
	500	0/4
	1000	0/4
	5000	0/4
AcTub^HI	100	0/4	1/1454 (1/3523-1/600)	0.0000539
	500	1/4
	1000	2/4
	5000	4/4
ALDH^HI	100	0/4	1/540 (1/1248-1/234)	0.00000045
	500	2/4
	1000	4/4
	5000	4/4

Both AcTub^HI and ALDH^HI isolated cells had significantly increased xenograft initiating frequency compared to control cells (**p<0.001). TIC frequency was calculated using the Extreme Limiting Dilution Analysis software (Hu and Smyth, 2009).