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. Author manuscript; available in PMC: 2014 Jun 26.
Published in final edited form as: Neuroscience. 2013 Oct 3;255:33–44. doi: 10.1016/j.neuroscience.2013.09.051

Figure 4. Confocal microscopy verifies that surface GABAAR α1 subunits are decreased by gephyrin siRNA on amygdala neurons.

Figure 4

Panel A is an example of control group, showing green-fluorescent α1 and red-fluorescent gephyrin, and merged picture. Panel B represents neurons treated with BDNF for 20 minutes. Both α1 and gephyrin puncta are decreased compared with Panel A control. Panel C shows cells transfected with pLV-siGephyrin, a gephyrin siRNA with GFP expression. The red-fluorescent α1 puncta are decreased. Panel D shows cells transfected with pLV-siGephyrin and treated with BDNF for 20 minutes. As in Panel C, the red-fluorescent α1puncta are greatly lowered. Panel E shows cells treated with BDNF for 1 hour, a time at which both gephyrin and α1puncta are markedly increased. Panel F shows cells transfected with pLV-siGephyrin and treated with BDNF for 1 hour. The red-fluorescent α1 subunits are largely decreased comparing with α1 in Panel E. Panel G is an example of cells transfected with pLV-siRNA control. The α1 and gephyrin expression is same as the control Panel A. (pLV-siG stands for pLV-siGephyrin).