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Effects of ciprofloxacin on Toxin A and B production by the C. difficile NAP1 039 strain. Soluble Toxin A and B measured by ELISA in the 64 µg ml−1-treated (0.25×MIC) culture. Ciprofloxacin was added at the final concentration during early exponential phase growth, designated time 0. Culture supernatant samples were collected over 48 h for detection of TcdA/TcdB by ELISA. Data shown are the means±sd of three replicates. •, No treatment; ○, 64 mg ml−1.
