Figure 4.

Cell migration assay. (a) The effect of human IgG, CTX, and M-CTX-Fc on the migration of PANC-1 cells was assessed using a PET track-etched membrane culture insert (pore size, 8.0 μm). 3 × 10⁴ cells were incubated with human IgG, CTX, and M-CTX-Fc in the range of 0–300 nM. Translocated cell numbers were normalized against those in the absence of human IgG, CTX, and M-CTX-Fc. The results are shown as mean ± SD from three independent experiments. (*P < 0.1, **P < 0.05, ***P < 0.01). (b) PANC-1 cells were seeded onto 12-well plates at a density of 3 × 10⁴ cells/well and cultured. After 20 h of culture, M-CTX-Fc at a range of 0–300 nM was added to each well for 48 h, and then the cells were trypsinized and counted.