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(a) Proliferation inhibition activity. Five thousand cells were grown in the presence of CTX and M-CTX-Fc for 48 h. (b) The viable cells at 48 h were kept cultured without CTX and M-CTX-Fc up to 96 h. Cell numbers in each well were assessed by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The absorbance at 570 nm corresponding to initial number of cells was defined as 1.
