Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: FEBS Lett. 2013 Sep 13;587(21):3456–3463. doi: 10.1016/j.febslet.2013.08.034

Figure 3. MPs from healthy subjects transfer miRNA to cultured endothelial cells more efficiently than MPs from CHD patients.

Figure 3

A) MP-mediated miRNA transfer to cultured HUVECs. HUVECs were incubated at 37°C for 2 h with MPs obtained from the sera of healthy subjects or with PBS (as control). After incubation, intracellular levels of miR-92a and miR-223 were quantified using qRT-PCR analysis. B) Impact of CHD on MP-mediated miRNA transfer. HUVECs were incubated with MP obtained from the sera of healthy subjects or patients with significant CHD. After incubation, HUVECs were harvested and intracellular miR-92a and miR-223 were assessed. The miRNA transfer index was calculated from qRT-PCR-based measurement of intracellular miRNA abundance, as described in Materials and Methods (Healthy n = 4, CHD n = 4 for panels A, B). #p < 0.05 (Student t test), *p < 0.05 (ANOVA, Bonferroni Posthoc).