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. Author manuscript; available in PMC: 2014 Feb 3.
Published in final edited form as: J Cereb Blood Flow Metab. 2009 Mar 25;29(6):1084–1098. doi: 10.1038/jcbfm.2009.32

Figure 4.

Figure 4

Expression of TJ protein in brain microvessels during CIP in the presence and absence of an ALK5 inhibitor. Western blot analysis of microvessels isolated from rats treated with saline or λ-carrageenan in the presence and absence of SB431542, a selective ALK5 inhibitor. Crude membrane preparations from rat brain microvessels (20 µg) were resolved on a 10% sodium dodecyl sulfate–polyacrylamide gel and transferred to a PVDF membrane. Samples were analyzed for expression of claudin-3 (A), claudin-5 (B), occludin (C), and ZO-1 (D). Claudin-3 was detected using the polyclonal antibody M-20 (1:500 dilution), claudin-5 was detected using the monoclonal antibody 4C3C2 (1:500 dilution), occludin was detected using the monoclonal antibody OC-3F10 (1:1000 dilution), and ZO-1 was detected using the polyclonal antibody H-300 (1:1000 dilution). Relative levels of TJ protein expression were determined by densitometric analysis. Results are expressed as mean ± s.d. of three separate experiments. Asterisks represent data points that are significantly different from control.